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EFFECT OF CENTRIFUGATION AND REPEATED WASHING ON THE FERTILIZING CAPACITY OF FOWL SPERMATOZOA

The technique of washing has been used to study various biological processes of mammalian (Lardy & Phillips, 1943; White, 1953a, b; Dott & Walton, 1960; Brackett, 1969) and avian (Wilcox, 1958; Wilcox & Clark, 1962) spermatozoa. In most of this work, the spermatozoa were diluted before washing. Emmens & Swyer (1948) concluded that the effects of washing and dilution were analogous and therefore dilution of the semen before centrifugation might be expected to mask the effect of washing. The object of this study was to examine the effects of centrifugation and repeated washing on the fertilizing capacity of the spermatozoa of the fowl, Gallus domesticus, without prior dilution.

Pooled semen was collected from Athens random-bred roosters by abdominal massage (Burrows & Quinn, 1935) and maintained at 41° C until insemination. Semen