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BIOCHEMICAL DETECTION AND ACTIVATION OF AN INACTIVE FORM OF A TRYPSIN-LIKE ENZYME IN RABBIT TESTES

The acrosomal trypsin-like enzyme of rabbit spermatozoa has been reported to be involved in sperm penetration of the zona pellucida (Stambaugh, Brackett & Mastroianni, 1969). It has also been suggested that seminal plasma trypsin inhibitors inhibit mammalian sperm trypsin-like activity until removed from spermatozoa during capacitation (Zaneveld, Srivastava & Williams, 1969). The present report describes the biochemical detection and activation of an inactive form of a trypsin-like enzyme in rabbit testis.

Trypsin-like activity was assayed by the spectrofluorometric detection of β-naphthylamine released from -benzoyl-DL-arginine-β-naphthylamide HCl (BANA, Schwarz, Mann Company), which is a specific substrate for trypsin and trypsin-like enzymes (Riedel & Wünsch, 1959). A Hitachi-Perkin-Elmer 2A spectrofluorometer was utilized for the assays which were carried out at 25° C with an excitation wavelength of 330 mµ

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				R. Stambaugh and M. Smith Amino Acid Content of Rabbit Acrosomal Proteinase and Its Similarity to Human Trypsin Science, November 22, 1974; 186(4165): 745 - 746. [Abstract] [PDF]