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PURIFICATION OF ASPERMATOGENIC SUBSTANCE IN BULL SEMINAL VESICLE FLUID

J. DOSTÁL and J. MATOUEK

Damage to spermatogenesis in mice, rats, guinea-pigs, rabbits and rams after the intramuscular or subcutaneous administration of bull seminal vesicle fluid was reported by Matouek (1966, 1969). Matouek & Petrovská (1969) also observed an inhibitory effect of bull seminal vesicle fluid on the fertility of female mice and hens. The present report deals with the separation and purification of a substance that causes aspermatogenesis in mice.

Seminal vesicle fluid obtained from mature bulls at slaughter was diluted 2·5-fold with 2% acetic acid and mixed for 30 min at room temperature. The precipitate was separated from the supernatant fluid by centrifugation at 7000 g for 45 min. The aspermatogenic substance remained in the supernatant fluid. The bulk of the protein was then precipitated by 3 M-ammonium sulphate and separated from the supernatant by further

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