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RAPIDITY OF REMOVAL IN VITRO OF THE CUMULUS OOPHORUS AND THE ZONA PELLUCIDA IN DIFFERENT STRAINS OF MICE

Summary.: The time needed for granulosa cell dispersal with hyaluronidase and for lysis of the zona pellucida with pronase and trypsin was estimated in vitro in fertilized and unfertilized eggs from four inbred strains, and one outbred strain of mice.

Granulosa cells were dispersed most rapidly in the C57 and most slowly in the CBA inbred strains. About 40% of the CBA ova remained undenuded after 2 hr treatment. The time needed for removal of the zona pellucida with both enzymes depended on the strain of the female and on the physiological state of the eggs. It was shortest in unfertilized eggs ovulated on the day of treatment, and longest in fertilized and in old fragmented eggs.

The zona reaction (assessed by the difference between fertilized and unfertilized eggs in the time needed for lysis of the zona) was most effective in CBA strain eggs and least effective in KE inbred strain eggs. These differences may explain the varying incidences of supplementary spermatozoa found in the fertilized eggs of the tested strains.

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