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Recent studies have demonstrated that a saturated aqueous solution of potassium pyroantimonate can be used as a precipitant of inorganic cations as well as a fixative (Tandler, Libanati & Sanchis, 1970; Schuchner & Tandler, 1972). Tissue fixed by this saturated solution alone (Tandler et al., 1970; Kierszenbaum, Libanati & Tandler, 1971; Schuchner & Tandler, 1972; Schuchner, Foix & Borenstein, 1972; Stockert & Schuchner, 1972) or by a potassium pyroantimonate/osmium tetroxide solution (Komnick, 1962; Spicer, Hardin & Greene, 1968; Hardin & Spicer, 1970) showed the presence of electron-opaque antimonate precipitates that could be analysed by microprobe. Analysis provided evidence that the precipitates represented such inorganic cations as Ca, Mg or Na (Tandler et al., 1970; Kierszenbaum et al., 1971). In the present study, the subcellular distribution ofinorganic cations was studied
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