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FURTHER CHARACTERIZATION OF THE PROTEINS OF BOAR SEMINAL PLASMA BY ISOELECTRIC FOCUSING ON POLYACRYLAMIDE

In an earlier study on the characteristics of boar seminal plasma (Boursnell, Nelson & Cole, 1966), use was made of ultrafiltrates, though this did not include an investigation of the ultrafiltered proteins. Recently, preliminary determinations have shown that the ultrafiltrate contains 4% of the seminal plasma protein and is devoid of the haemagglutinating protein H (Boursnell & Briggs, 1969).

In a further study of the nature of this protein material, isoelectric focusing on polyacrylamide plates was used. Comparisons of this material were made with whole seminal plasma, seminal plasma from which the haemagglutinin was absorbed with washed bull spermatozoa (Boursnell, 1967) and with proteins `A Seph' and `A Dial' prepared from boar seminal plasma as described by Boursnell et al. (1966). `A Seph' should contain the haemagglutinin and `A Dial' should lack it (Boursnell