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FIRST ATTEMPT TO CHARACTERIZE AN ASPERMATOGENIC SUBSTANCE IN THE SEMINAL VESICLE FLUIDS OF BULLS

J. MATOUEK, R. STANK and L. VESELSK

Summary.: The biosynthesis of the aspermatogenic substance in the seminal vesicle fluid of bulls is dependent on the androgen level. The aspermatogenic substance is not present in sexually immature bulls; its synthesis can be evoked, however, by means of testosterone propionate. Castration is followed by a loss of libido and a decline in the production of fluids from the accessory genital glands; injections of testosterone propionate restore libido, the secretion of fluids and their aspermatogenic effect.

The aspermatogenic substance is thermostable, it is not digested by a number of enzymes including proteolytic enzymes, but it must evidently be bound to a protein complex in order to induce aspermatogenesis.

Rabbits developed several antibodies against bull vesicular fluid treated with enzymes, as judged by double diffusion, immunoelectrophoresis and haemagglutination tests. The number and character of the precipitation and haemagglutination antibodies in immunized animals did not differ whether the testes were damaged or undamaged. The fluorescence technique did not detect any binding of antibodies either to epididymal spermatozoa or to histological sections of the testes of rabbits and bulls.