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ALKALINE PHOSPHATASE IN THE CYTOPLASMIC DROPLET OF MAMMALIAN SPERMATOZOA

Summary.: Alkaline phosphatase activity has been examined in ram, rabbit, hamster and rat spermatozoa. Results have shown that the enzyme is restricted to the cytoplasmic droplet in rabbit and ram spermatozoa but that the intensity of reaction is highest in rabbit spermatozoa. The enzyme could not be demonstrated at all in the spermatozoa of the hamster or rat.

It has also been shown that alkaline phosphatase activity in the droplet of rabbit spermatozoa decreases during epididymal transit and that there is a reduction in activity in degenerating spermatozoa. Degeneration in spermatozoa was produced in rabbits by means of artificial cryptorchidism, and after 2 weeks, activity in the droplets was completely lost.