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RADIOIMMUNOASSAY OF LUTEINIZING HORMONE IN THE BLOOD OF ZEBU CATTLE

Summary.: A solid-phase radioimmunoassay was used to measure LH in the serum of a Zebu cow and heifer during the oestrous cycle. Shortly after the onset of oestrus, serum levels of LH rose sharply to 20 to 120 ng/ml before dropping to baseline levels of about 2 ng/ml after 8 to 10 hr. Elevated LH values were also detected in mid-cycle, although considerable variation in activity over several days occurred with the heifer. Peak values of LH were considerably higher in the serum of the cow than the heifer.