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HISTOCHEMICAL LOCALIZATION OF 3βHYDROXYSTEROID DEHYDROGENASE IN THE RAT EPIDIDYMIS

Recent studies have demonstrated that cholesterol and testosterone may be synthesized in vitro from acetate by mouse and rat epididymides (Hamilton, Jones & Fawcett, 1969; Hamilton & Fawcett, 1970). McGadey, Baillie & Ferguson (1966) were the first to demonstrate histochemically some of the steroid-metabolizing enzymes in the hamster epididymis, and subsequently a number of workers (Gloyna & Wilson, 1969; Inano, Machino & Tamaoki, 1969) have verified by biochemical means the existence of steroid-converting enzymes in the epididymal tissue of a number of mammals. McGadey et al. (1966), however, did not find 3β-hydroxysteroid dehydrogenase in the hamster epididymis. This enzyme is very much concerned with steroid biosynthesis, converting pregnenolone and dehydroepiandrosterone (DHA) to progesterone and androstenedione, respectively (Baillie, Ferguson & Hart, 1966). In many vertebrates, steroid-producing organs