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VOLUME AND PROTEIN CONCENTRATION OF RABBIT UTERINE FLUID

Summary.: Uterine lumina of rabbits were rinsed in situ with BSA solution. Uterine fluid volume and luminal protein concentration were calculated from radial immunodiffusion and biuret tests of the rinse. The procedure was checked for three sources of error and confirmed by double rinses.

Non-pregnant and lactating rabbits have nearly equal amounts of protein per horn, but different volumes of fluid. With pregnancy, increase of volume occurs without proportionate increase of protein. Peak protein concentrations occur in pregnant and pseudopregnant rabbits at 5 days p.c. Volume and protein concentration of the luminal fluid appear to be sensitive indicators of the hormonal and reproductive status of the uterus.

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