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COLLECTION OF OVIDUCT FLUID FROM UNRESTRICTED MONKEYS

Hamner & Fox (1969) have recently reviewed various techniques for the collection of oviduct fluid in the rabbit, sheep, pig and monkey. Continual collection of the oviduct fluid from the rhesus monkey was first achieved by Mastroianni, Shah & Abdul-Karim (1961). They cannulated the oviducts with silicone rubber tubings which were connected to an external volumetric collection device fixed to the skin. Movements of the animals' upper limbs were restricted with plaster to prevent the animals from reaching the device. Using this technique, daily samples were collected for as long as four complete menstrual cycles.

In view of the possibility that a physical restriction of the monkey might cause a deleterious effect on the normal secretory patterns of the fluid, we developed a device which allows daily collection of the fluid from unrestricted monkeys. The device was installed intra-abdominally and samples were taken for a period of over 3 months, after which, however, the device was subject to rejection by the animal. The technique and preliminary data obtained are described in this report.