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THE `SPERM-RISE' TEST

After centrifugation of a mobile-sperm suspension, the spermatozoa will begin to move upwards from the sediment into the supernatant. This fact constitutes the background to the following method for comparative estimation of the `amounts of motility' in different test suspensions. The method is simple, adequately objective, and recordings can be made in parallel on several different test suspensions in which the spermatozoa have been in contact with various media for an identical time.

Test suspensions containing 0·5% of cells (see below) were prepared from well-mixed stock suspensions of spermatozoa pooled from the cauda epididymidis of a varying number of gonads from slaughtered bulls. When the effects of different drugs upon sperm kinetic activity were studied, the suspending medium was a Ringer solution in which the 100-ml 0·9% NaCl (0·154 M) component of Mann's