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INCORPORATION OF [U-¹⁴C]PALMITIC ACID INTO THE PHOSPHOLIPIDS OF BOVINE SEMEN

Fatty acids play a major rôle in the metabolism of spermatozoa. The endogenous respiration of these cells depends largely on the oxidation of hydrolysed acyl groups of plasmalogens (Hartree & Mann, 1961); endogenous fatty acids may also be utilized and are readily incorporated into the lipids of spermatozoa (Payne & Masters, 1968; Mills & Scott, 1969). This communication was initiated in order to provide further data on the turnover of individual phospholipids, and constitutes a preliminary report on the rate and specificity of incorporation of palmitic acid into the phosphatides of bovine semen.

Samples (1-ml) of freshly ejaculated semen were incubated under aerobic conditions at 32° C with [U-¹⁴C]palmitic acid (0·5 µC) in Krebs-Ringer phosphate buffer (pH 7·4 containing 1·1 ml of 3% bovine serum albumin (Calbiochem,

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				I. Thérien, R. Moreau, and P. Manjunath Bovine Seminal Plasma Phospholipid-Binding Proteins Stimulate Phospholipid Efflux from Epididymal Sperm Biol Reprod, September 1, 1999; 61(3): 590 - 598. [Abstract] [Full Text]