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INHIBITION IN VITRO OF INCORPORATION OF [³²P]PHOSPHATE INTO RABBIT AND HUMAN ENDOMETRIAL PHOSPHOLIPIDS

Summary.: Endometria from rabbits and humans were incubated with [³²P]phosphate and several physiological or synthetic amino-alcohols. Sphingomyelin, phosphatidyl choline, phosphatidyl serine and phosphatidyl ethanolamine were then isolated and their specific radioactivities determined. Addition of choline to the media resulted in an increased incorporation of ³²P into phosphatidyl choline; neither ethanolamine nor serine had any significant effect on any of the phospholipid classes. Aminomethylpropanol, 3-aminopropanol, triethanolamine, and dimethylaminoisopropanol in concentrations of 0·001 M to 0·1 M resulted in inhibiton of ³²P incorporation into all the rabbit endometrial phospholipid classes studied; the first three compounds had a proportionately greater effect upon phosphatidyl choline, whereas dimethylaminoisopropanol had a more uniform inhibitory effect on all the phospholipids. Methyl serine added to the media had no significant effect on incorporation of ³²P into any of the phospholipid classes.

Incubation of human endometria with 0·01-M concentrations of the above amino-alcohols resulted in similar inhibitory effects on ³²P incorporation into the phospholipid classes studied.