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Epididymal spermatozoa of the golden hamster can be capacitated in vitro in the presence of oviduct fluid from the oestrous female hamster (Yanagimachi & Chang, 1964; Yanagimachi, 1966; Barros & Austin, 1967), follicular fluid from mature ovarian follicles of the hamster (Barros & Austin, 1967; Yanagimachi, 1969a), detoxified bovine follicular fluid (Gwatkin & Andersen, 1969; Yanagimachi, 1969b), and detoxified blood sera of the hamster and some other species (Yanagimachi, 1970). When incubated in media containing these biological fluids, the spermatozoa agglutinate head to head within
hr. About 2
to 3 hr later, agglutinated spermatozoa disperse spontaneously and free spermatozoa show an extraordinarily active movement. In the majority (sometimes, 100%) of these spermatozoa, the acrosomal reaction is in progress or has been completed and the spermatozoa are ready to penetrate the
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