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ELECTROPHORETIC DETECTION OF MULTIPLE FORMS OF TRYPSIN-LIKE ACTIVITY IN SPERMATOZOA OF THE DOMESTIC FOWL

Buruiana (1956) reported the presence of trypsin activity in the spermatozoa of several species, including the cock. Recently Stambaugh & Buckley (1969) demonstrated that the trypsin-like enzymatic activity of rabbit sperm acrosomes could remove the zona pellucida of rabbit ova.

The present study is concerned with the electrophoretic detection of multiple forms of trypsin-like enzymatic activity present in ejaculated spermatozoa of the domestic fowl, Gallus domesticus.

Single-combed White Leghorn and Black Australorp cock semen was collected by abdominal massage, pooled, diluted with an equal volume of phosphate buffer (Wilcox, 1958), and centrifuged at 1200g for 10 min. The sperm deposit was washed three times with phosphate buffer, re-suspended in an equal volume of water containing glass beads and sonicated for 10 min with a model 185D sonifier (Heat Systems, Inc.) at an