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Journal of Reproduction and Fertility (1970) 22 371-373
DOI: 10.1530/jrf.0.0220371
Copyright © 1970 Society for Reproduction and Fertility
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ALKALINE PHOSPHATASE IN THE CYTOPLASMIC DROPLET OF RABBIT SPERMATOZOA

S. BAVDEK and T. D. GLOVER

The presence of acid and alkaline phosphatase has been demonstrated biochemically in bull spermatozoa (Roussel & Stallcup, 1966), and lysosomal acid phosphatases have been shown to occur in the cytoplasmic droplet of bull and ram spermatozoa (Dott & Dingle, 1968). Both types of phosphatases have also been shown histochemically in the spermatozoa of bulls (Krause, 1969) and several other mammalian species (Wislocki, 1949; Bern, 1949). Allison & Hartree (1969) have referred to acid phosphatase being concentrated in the acrosome of vertebrate and invertebrate spermatozoa but there appears to be no comprehensive information about the localization of alkaline phosphatase in spermatozoa. The present paper reports on such a localization in rabbit epidiymal spermatozoa.

During an investigation into the histochemistry of the rabbit epididymis, it was noticed that the cytoplasmic droplets of epididymal spermatozoa reacted positively when stained with







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