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USE OF A CONTINUOUS FLOW DIALYSIS APPARATUS TO DETERMINE THE EFFECT OF CATIONS, BUFFERS AND OSMOTIC PRESSURE ON RAM SPERMATOZOA

Summary.: When ram semen was dialysed against phosphate-glucose diluents with a cation composition similar to rete testis fluid, epididymal and ejaculated seminal plasma and ejaculated ram spermatozoa, the impedance change frequency (ICF) was maintained best over 6 hr in the latter diluent. Krebs-Ringer-glucose buffered with sodium phosphate or trisodium citrate maintained the ICF at a high level, whereas tris-HCl and veronal-HCl buffers caused a rapid decline in ICF. Hypertonic dialysis media severely depressed the ICF within 1 hr, whereas hypotonic diluents gave results similar to Krebs-Ringer-phosphate.

Adding 1 mM-calcium or 5 to 51 mM-potassium to an isotonic dialysis fluid was beneficial and the effects on ICF were additive. The omission of potassium from the hypotonic diluent caused no significant reduction in ICF.