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Summary.: Glutamate concentration and total content were determined in the post-natal rat testis by an enzymatic method. The concentration of glutamate in the rat testis was found to be higher than had been reported in ram testicular fluid, but about the same as in human seminal plasma. A significant increase in concentration occurred between 15 and 20 days of age. It coincided with the onset of fluid secretion by the seminiferous tubules and spermatogenesis.
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