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FACTORS AFFECTING THE AMOUNT AND SITE OF ACCUMULATION OF ACETATE AND LACTATE BY RAM SPERMATOZOA

Summary.: The amount of acetate accumulated from lactate by ram spermatozoa during incubation at 37° C was increased by storage of semen at 5° C and by incubating washed cells in diluents without added potassium and magnesium ions.

When ram semen was incubated shortly after ejaculation, 70% of the acetate accumulated was intracellular. After storage overnight at – 79° C, only 10% of the acetate accumulated during incubation was intracellular.

The intracellular acetate was not extracted from the cell by hexane: butanol mixtures but was removed by extraction with 2·% perchloric acid.