This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by PEDERSEN, T. Search for Related Content PubMed Articles by PEDERSEN, T.

DETERMINATION OF FOLLICLE GROWTH RATE IN THE OVARY OF THE IMMATURE MOUSE

Summary.: The cell population kinetics of the ovary in 28-day-old mice were investigated. A method to determine growth rates of follicles is described, and the results obtained are discussed.

The granulosa cells in each follicle were considered to represent a single cell population. The time taken to double such a cell population was calculated from the labelling index of the granulosa cells and the duration of the synthesis phase of the single cells. Both parameters have been determined in autoradiographs prepared at different time intervals after the injection of tritiated thymidine.

Different stages of follicle development were classified according to the number of their granulosa cells. The actual time taken to grow to different stages was calculated from the time taken to double the number of the granulosa cells in these stages. It was shown that medium follicles grow at a slower rate than large follicles. A small follicle with one layer of granulosa cells took 16 days to develop into a large one with several layers and antrum formation.

This article has been cited by other articles:

				M.Y. Yang and J.E. Fortune The Capacity of Primordial Follicles in Fetal Bovine Ovaries to Initiate Growth In Vitro Develops During Mid-Gestation and Is Associated with Meiotic Arrest of Oocytes Biol Reprod, June 1, 2008; 78(6): 1153 - 1161. [Abstract] [Full Text] [PDF]

				F. Sun, I. Betzendahl, Y. Shen, R. Cortvrindt, J. Smitz, and U. Eichenlaub-Ritter Preantral follicle culture as a novel in vitro assay in reproductive toxicology testing in mammalian oocytes Mutagenesis, January 1, 2004; 19(1): 13 - 25. [Abstract] [Full Text] [PDF]

				L. M. Mitchell, C. R. Kennedy, and G. M. Hartshorne Effects of varying gonadotrophin dose and timing on antrum formation and ovulation efficiency of mouse follicles in vitro Hum. Reprod., May 1, 2002; 17(5): 1181 - 1188. [Abstract] [Full Text] [PDF]

				A. L. L. Durlinger, P. Kramer, B. Karels, F. H. de Jong, J. Th. J. Uilenbroek, J. A. Grootegoed, and A. P. N. Themmen Control of Primordial Follicle Recruitment by Anti-Mullerian Hormone in the Mouse Ovary Endocrinology, December 1, 1999; 140(12): 5789 - 5796. [Abstract] [Full Text]