This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by HEALEY, P. Articles by WEIR, B. J. Search for Related Content PubMed Articles by HEALEY, P. Articles by WEIR, B. J.

CHANGES IN THE ULTRASTRUCTURE OF CHINCHILLA SPERMATOZOA IN DIFFERENT DILUENTS

During the search for a suitable medium in which to freeze spermatozoa of the chinchilla (Chinchilla laniger), samples taken from ten males by electro-ejaculation (Healey & Weir, 1967) were pooled and studied after treatment with different diluents. All the samples contained a large proportion of motile spermatozoa and no samples were discarded before treatment.

One aliquot of undiluted semen was dropped into 2 % buffered osmium tetroxide at pH 7·4 (Palade, 1952) and other aliquots were diluted in 0·5 ml of Baker's fluid (Baker, 1931), normal Ringer (8·5 g sodium chloride, 0·42 g potassium chloride and 0·25 g calcium chloride/litre water), half-strength Ringer or double-strength Ringer. The undiluted semen solidified immediately in the fixative and only the outer layer was used. The sperm suspensions in the different diluents were left at room temperature