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THE CHARACTERIZATION OF GLUCOSE-6-PHOSPHATE DEHYDROGENASE IN MOUSE OVA

Previous studies have indicated that the glucose-6-phosphate dehydrogenase (G6PD) contained within mouse ova is synthesized before ovulation and is under the genetic control of the X-chromosome (Epstein, Wegienka & Smith, 1969; Epstein, 1969). As such, it is different from the autosomally controlled hexose-6-phosphate dehydrogenase (H6PD) or glucose dehydrogenase found in liver microsomes but not in erythrocytes (Beutler & Morrison, 1967). To establish the applicability of these genetic results with ova to other tissues of the mouse, G6PD from ova has been characterized and compared with erythrocyte G6PD.

Ova and pre-implantation embryos were obtained from Swiss albino mice (Mus musculus) by superovulation. For microspectrophotometric assay of enzyme activity, they were lysed by freezing and thawing in 0·05 M-tris buffer, pH 7·5, containing 0·05% bovine serum albumin (BSA) (Epstein et al., 1969). For polyacrylamide disc