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A METHOD FOR PREPARING SINGLE MAMMALIAN OVA FOR CYTOLOGICAL STUDIES USING LIGHT AND ELECTRON MICROSCOPY

The inability to define accurately the cytological characteristics associated with fertile mammalian ova is due, at least in part, to difficulty in obtaining and preparing adequate numbers of these cells for critical morphological studies. This communication describes a method which has proved useful in preparing single bovine ova for study using both light and electron microscopy.

Bovine ova were recovered by follicular aspiration immediately following slaughter. Aspiration was accomplished with a 20-gauge hypodermic needle and a 2-ml syringe (Pl. 1, Fig. 1). The follicular fluid was placed in a watch-glass (Pl. 1, Fig. 2) and scanned, using a stereomicroscope in order to locate the ovum. Following location, the ovum was transferred with a micro-pipette from the follicular fluid to a fixation ampoule suspended in an ice water bath (Pl. 1, Fig. 3). The fixation ampoules were