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CULTURE OF TWO-CELL RABBIT EMBRYOS TO MORULAE

Summary.: The rabbit embryo is able to develop from the two-cell stage to the morula in a medium containing an amino-nitrogen source as the only metabolizable component. This amino-nitrogen source can be bovine serum albumin, oxidized glutathione, or certain single amino acids such as glutamine, proline or alanine. Although a requirement for a non-nitrogenous energy source could not be demonstrated during this period, a beneficial effect of pyruvate and lactate was shown. The optimum concentration for pyruvate was approximately 10 _–4 M and for lactate was 1 to 3x10_–2M.