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Summary.: Three methods were used for the detection of ovulation in twenty-five cycles of sixteen monkeys of the species Macaca irus: (1) rectal palpation of the urogenital tract, (2) the karyopycnotic index (KPI) of the vaginal smear cytology, and (3) a simple chemical test for the apparent changes in the chloride content of the vaginal mucus.
Daily vaginal smears were necessary to indicate the first day of the menstrual cycle as, in some animals, menstruation began as an occult flow lasting 1 to 3 days. Rectal palpation of the uterus and ovaries gave the precise time of ovulation and was the only method which would differentiate, with certainty, between ovulation and anovulation.
Both KPI and the vaginal mucus chloride test failed to indicate accurately the time of ovulation, and in some cases gave a false indication of ovulation, but the chloride test did give several days' warning that ovulation was imminent.
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