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Journal of Reproduction and Fertility (1970) 21 119-126
DOI: 10.1530/jrf.0.0210119
Copyright © 1970 Society for Reproduction and Fertility
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FACTORS AFFECTING SUPEROVULATION, FERTILIZATION AND RECOVERY OF SUPEROVULATED OVA IN PREPUBERAL CATTLE

HIDEO ONUMA, J. HAHN and R. H. FOOTE

Summary.: Holstein calves, reared from birth under standard conditions, were given various combinations of PMSG-LH or PMSG-HCG at 8 to 9 weeks, or at 17 weeks of age. Follicular development was variable, but averaged fifty-three follicles≥1·0 cm in diameter following administration of either 1500 or 2000 i.u. of PMSG. Ovulation rate was 79% following 50 mg of LH, but it was only 16% following 1500 i.u. of HCG. The presence of young corpora lutea or the administration of progesterone accelerated transport of ova into the uterus and was associated with a reduced percentage of cleaved ova and a lower recovery rate. Liquid semen was superior to frozen semen in initiating cleavage of ova. Under optimum conditions, approximately 75% of the developed follicles ovulated and a similar proportion of ova was cleaved at the time of collection or cleaved in culture. Recovery rate was usually less than 30%.




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G. Seidel Jr
Superovulation and embryo transfer in cattle
Science, January 23, 1981; 211(4480): 351 - 358.
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Copyright © 1970 by the Society for Reproduction and Fertility.