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DIFFERENTIATION OF RABBIT SPERM ANTIGENS FROM THOSE OF SEMINAL PLASMA

Summary.: Sperm-specific and sperm-coating antigens in rabbit seminal constituents were studied using agar-gel diffusion and immuno-electrophoresis. The antigenicity of ejaculated rabbit spermatozoa was due to two glycoprotein sperm-specific antigens of testicular origin and twelve seminal plasma antigens which coated the spermatozoa during passage through the reproductive tract. Two of the sperm-coating antigens originated in the testis, two in the epididymis and eight above the level of the vas deferens. Relationships between sperm-coating antigens and capacitation, and sperm-coating antigens and prevention of immunologically induced aspermatogenesis or immunologically induced infertility in the female were proposed.