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RESPIRATORY STUDIES OF BOVINE SPERMATOZOA AND ENDOMETRIAL PREPARATIONS

Summary.: Bovine spermatozoa, washed or in seminal plasma, were incubated alone or in combination with oestrogenic and progestational endometrial preparations; oxygen consumption was determined for 1 hr.

Oxygen uptake by combinations of washed spermatozoa and endometrial preparations could be predicted by addition of the respiratory activity of the isolated tissues. When diluted whole semen was substituted for washed spermatozoa, oxygen uptake by the combined tissues was significantly less than that predicted by addition. The factor in seminal plasma responsible for the depression of respiratory activity was not identified.