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AMANTADINE HYDROCHLORIDE: INHIBITOR OF CLEAVAGE AND DNA SYNTHESIS IN SEA URCHIN OVA

Amantadine hydrochloride (Symmetrel, E. I. Du Pont de Nemours and Co.), prevents entry of some influenza viruses into host cells (Hoffmann, Newmayer, Haff & Goldsby, 1965). Since sperm penetration into mammalian ova may be accomplished by mechanisms similar to those of virus penetration (Soupart & Noyes, 1964; Soupart & Clewe, 1965), we have investigated the effects of amantadine on a model fertilization system, the egg of the sea urchin, Arbacia punctulata. In these ova, a fertilization membrane is observed within 2 min after mixing spermatozoa and eggs, syngamy and first cleavage are completed within 15 and 70 min, respectively, at 23° C and pH 8·2 in artificial sea water (Harvey, 1956).

Gametes were collected by electrical stimulation, and the jelly coat of eggs was removed with HCl (Harvey,