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ESTIMATION OF SPERMATOZOA NUMBERS USING A MICROPORE FILTRATION TECHNIQUE

The estimation of sperm concentration of semen samples has been accomplished by a variety of different methods. The equipment used in these techniques has ranged from the simple haemocytometer chamber to more highly sophisticated electronic means such as measuring light transmission (Willet & Buckner, 1951) and counting particle size (Glover & Phipps, 1962). Although electronic means of counting spermatozoa offer obvious advantages in situations where large numbers of routine counts are involved, estimates made with a haemocytometer have been the method of choice for most researchers because the technique is relatively accurate, utilizes simple inexpensive equipment and is rapid. In addition, the haemocytometer, unlike electronic methods, has been used successfully in conjunction with centrifugation to study the movement of spermatozoa through the female reproductive tract (Braden, 1953). This method, however, was not entirely satisfactory in our laboratory when it was applied to reproductive studies involving pharmacological means of impeding