This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by FREUND, M. Search for Related Content PubMed PubMed Citation Articles by FREUND, M.

INTERRELATIONSHIPS AMONG THE CHARACTERISTICS OF GUINEA-PIG SEMEN COLLECTED BY ELECTRO-EJACULATION

Summary.: Methods and equipment for collection of guinea-pig semen by electro-ejaculation and for liquefaction of coagulated semen ('rodent vaginal plug') with chymotrypsin are described. The electro-ejaculator was designed to deliver a 1000-cycle 12-volt square wave, via ano-lumbar (L1 to L4) pathways, with an automatic 3-sec `on period' and 12-sec `off period'. Thirty semen specimens were collected at weekly intervals from each of twenty-nine unselected guinea-pigs. Means for semen characteristics of the 870 specimens were: sperm concentration per ejaculate, 13·376x 10⁶; ejaculate volume, 0·5 ml; percentage motility, 66%; percentage normal morphology, 95%. Mean fructose concentration was 102 mg%. Oxygen uptake by the spermatozoa was large and the ZO₂ (µl/10⁸ spermatozoa/hr) was calculated to be 25·03. Stimulation of vertebral levels L1 to L4 simultaneously resulted in ejaculation without erection while stimulation of any one of levels L5, L6, S1, S2 resulted in erection. The method described appears to be a precise, repeatable and non-traumatic method of electro-ejaculation which is useful in studies to determine the effects of radiation or drugs on sperm production in the guinea-pig.

This article has been cited by other articles:

				R. G. Rank, A. K. Bowlin, R. L. Reed, and T. Darville Characterization of Chlamydial Genital Infection Resulting from Sexual Transmission from Male to Female Guinea Pigs and Determination of Infectious Dose Infect. Immun., November 1, 2003; 71(11): 6148 - 6154. [Abstract] [Full Text] [PDF]