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A QUANTITATIVE IN VITRO RESPONSE TO FOLLICLE-STIMULATING HORMONE

Summary.: A series of six factorial experiments is described in which ovaries of 15-day-old mice were cultured in vitro for 2 to 4 days in the presence of various concentrations of highly purified FSH and LH. The uptake of [¹⁴C]thymidine by the tissue was measured in all experiments. FSH consistently and significantly increased the uptake where the mice used were above a certain size. In only two experiments did LH significantly increase thymidine uptake; this effect appeared to depend on certain precise conditions of culture. The uptake of [³H]lysine was also measured in two of the six experiments; it was significantly increased by FSH in one of them. LH had no significant effect on lysine uptake. There were no significant interactions between FSH and LH. It is concluded that the uptake of labelled thymidine in response to FSH should have useful experimental applications. The possibility that the exclusive role of FSH is to initiate DNA synthesis in follicle cells is briefly discussed.

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