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LUTEINIZING HORMONE SECRETION DURING THE OESTROUS CYCLE OF THE EWE AS DETERMINED BY RADIO-IMMUNOASSAY

This note presents preliminary results on the use of a radio-immunoassay method involving the use of¹²⁵I LH prepared by the method of Greenwood, Hunter & Glover (1963) for the determination of LH in the blood of ewes throughout the oestrous cycle. Particular attention was paid to the period around oestrus. In the assay, 0·3 ml plasma is incubated for 72 hr at 4° C with 100 pg¹²⁵I LH (100 µc/µg, vol 0·1 ml in phosphate buffer, 0·05 M, pH 7·4, containing 0·25% bovine serum albumin, Fraction V) and with 1·0 ml of 1:250,000 LH antiserum, also diluted in phosphate buffer. After incubation, during which the¹²⁵I LH and LH present in plasma compete for binding to the antiserum, 150 mg talc is