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Summary.: The haemagglutinating protein (or proteins) have been separated from boar seminal plasma largely by gel filtration methods. Electrophoretic studies have shown that the haemagglutinating protein material (now designated Protein H) is positively charged and that it possesses an isoelectric point (i.e.p.) of about 9·4. Earlier electrophoretic observations on mixtures of boar seminal plasma proteins have been verified with the greatly purified Protein A (i.e.p. 8·8) and Protein B (i.e.p. 4·6). It has been possible to explain some hitherto puzzling properties of the boar seminal plasma caused by isolation of various combinations of Protein A, Protein B and Protein H.
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