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EFFECT OF NUCLEOSIDES AND NUCLEOSIDE BASES ON THE DEVELOPMENT OF PRE-IMPLANTATION MOUSE EMBRYOS IN VITRO

The nutritional requirements for the development in vitro of 2-celled mouse embryos into blastocysts have been extensively investigated by Brinster (1965a, b, c). It was found that most 2-celled embryos will develop normally in a culture medium consisting of bicarbonate-buffered Krebs-Ringer solution, containing bovine serum albumin as a protein source, and pyruvate and lactate as energy sources. Since there are always some 2-celled embryos which will not develop into blastocysts in this medium, the possibility exists that some other growth factors might increase the percentage of normally developing embryos. It is known that synthesis of DNA takes place throughout the cleavage stages and that RNA synthesis also occurs, especially after the 4-cell stage (Mintz, 1964). Thus it was thought that the addition of nucleic acid components