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Journal of Reproduction and Fertility (1968) 17 219-220
DOI: 10.1530/jrf.0.0170219
Copyright © 1968 Society for Reproduction and Fertility
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THE HIGH CONCENTRATION OF FREE MYO-INOSITOL IN RETE TESTIS FLUID FROM RAMS

B. P. SETCHELL, R. M. C. DAWSON and R. W. WHITE

A high concentration of free inositol has been found in the semen of a number of species and most of this seems to arise from the accessory glands, not the epididymis (Mann, 1954, 1964; Hartree, 1957). However, appreciable concentrations of myo-inositol occur in the testis (Dawson & Freinkel, 1961), and recent evidence suggests that it is most likely formed there from glucose via glucose-6-phosphate and D-myo-inositol-l-phosphate (Eisenberg, 1967). This high concentration in the testis prompted us to examine the levels of free inositol in rete testis fluid.

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Fluid was collected from five conscious Clun Forest rams by the technique of Voglmayr, Scott, Setchell & Waites (1967) and analysed for free inositol by the method of Campling & Nixon (1954) following deproteinization with perchloric acid (Dawson & Freinkel, 1961). The concentration of inositol was over 100 times that




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T. R. Chauvin and M. D. Griswold
Characterization of the Expression and Regulation of Genes Necessary for myo-Inositol Biosynthesis and Transport in the Seminiferous Epithelium
Biol Reprod, March 1, 2004; 70(3): 744 - 751.
[Abstract] [Full Text] [PDF]




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