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A high concentration of free inositol has been found in the semen of a number of species and most of this seems to arise from the accessory glands, not the epididymis (Mann, 1954, 1964; Hartree, 1957). However, appreciable concentrations of myo-inositol occur in the testis (Dawson & Freinkel, 1961), and recent evidence suggests that it is most likely formed there from glucose via glucose-6-phosphate and D-myo-inositol-l-phosphate (Eisenberg, 1967). This high concentration in the testis prompted us to examine the levels of free inositol in rete testis fluid.
Fluid was collected from five conscious Clun Forest rams by the technique of Voglmayr, Scott, Setchell & Waites (1967) and analysed for free inositol by the method of Campling & Nixon (1954) following deproteinization with perchloric acid (Dawson & Freinkel, 1961). The concentration of inositol was over 100 times that
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T. R. Chauvin and M. D. Griswold Characterization of the Expression and Regulation of Genes Necessary for myo-Inositol Biosynthesis and Transport in the Seminiferous Epithelium Biol Reprod, March 1, 2004; 70(3): 744 - 751. [Abstract] [Full Text] [PDF] |
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