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Summary.: Four electrophoretic experiments on rabbit spermatozoa were carried out using Afif, phosphate, Luck's phosphate, AFY, AFYAM and AFAM buffers. A new technique using motile spermatozoa and intermittent electric power was employed with three- and seven-chambered electrophoretic cells.
Two-way migration of spermatozoa was achieved. Sex ratios of offspring resulting from anodic and cathodic spermatozoa diluted in Afif, phosphate and Luck's phosphate buffers in the first experiment were significantly different from the expected normal sex ratio, while the other three experiments, in the main, did not show the same trend.
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