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COMPARISONS OF METHODS FOR LOCATING OVA IN THE OVIDUCT OF THE RABBIT

Summary.: A new technique for the location of ova in the rabbit oviduct is presented. The method consists of freezing the oviducts in situ immediately after killing the rabbits, and then removing and clearing them with benzyl-benzoate as described by Orsini (1962). The ova are located in the cleared oviducts under a dissecting microscope. The results obtained by this method are compared with the segmentation technique often used. When the position of ova in frozen-cleared oviducts was compared with the position of ova in segmented oviducts, ova were located approximately 10% further down the tube from 24 to 48 hr post coitum, at the same position at 60 hr and 10% closer to the ovarian end at 72 hr than when the segmentation method was used. Clearing alone resulted in the ova being located intermediate to the other two methods. It was concluded that the method by which ova are located in the oviduct has considerable bearing on the results obtained.