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LABELLING OF SEMEN WITH RADIO-ACTIVE PHOSPHATE

The purpose of the preliminary experiments described below was to label mammalian spermatozoa with ³²P to such a degree that they could afterwards be used for a quantitative follow-up of the so-called leakage of intracellular sperm compounds, such as is known to accompany sperm 'ageing' or 'senescence' (Mann, 1964). The approach was twofold, by means of labelling in vivo and in vitro.

The in-vivo study, in which four buck rabbits were injected subcutaneously with inorganic [³²p]phosphate (1 mc/animal), included radio-activity assays by liquid scintillation counting of urine, blood plasma, blood corpuscles, seminal plasma and spermatozoa. In the urine and blood plasma, specific radio-activity, high at first, declined rapidly, reaching a very low level by the end of the first month. In the blood corpuscles, there was first a decline in radio-activity which, however,