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HISTOCHEMICAL DEMONSTRATION OF ⁵-3β-AND 17β-HYDROXYSTEROID DEHYDROGENASES IN THE TESTES OF THE BOAR

Histochemical methods have been widely used for the detection and localization of hydroxysteroid dehydrogenase activity, mainly in steroid-hormone producing organs and tissues (Baillie, Ferguson & Hart, 1966). Hay & Deane (1966) attempted to demonstrate ⁵-3 β- and 17 β-hydroxysteroid dehydrogenases in testicular tissue of the larger farm animals. In the adult boar, an intense staining reaction in the interstitial tissue of the testes, incubated without substrate added to the medium, prevented the histochemical demonstration of a specific reaction for ⁵-3 β-hydroxysteroid dehydrogenase. In view of the high production of both oestrogens and androgens by the adult males of this species (Raeside, 1965), it seemed of particular interest to be able to apply methods for the detection of hydroxysteroid dehydrogenase activity to testicular tissue of the adult boar.

Testes were obtained from two adult boars