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EFFECT OF OESTROGEN AND PROGESTERONE ON PHYSICAL PROPERTIES OF RABBIT OVIDUCT FLUID

Since oviduct fluid acts as a medium for spermatozoa and ova during fertilization and early cleavage, this investigation was undertaken to determine any changes that occur in specific gravity, viscosity, osmolarity or dry matter content of rabbit oviduct fluid as the hormonal state changes from oestrogen to progesterone domination after fertilization.

Five New Zealand white rabbits (4·5 to 5·0 kg) were ovariectomized and oviduct fluid collecting flasks were installed (Hamner & Williams, 1965). The animals were injected subcutaneously with 1·0 µg oestradiol cypionate (OCP) /kg/day in 1·0 ml cottonseed oil (CSO) for 7 days, 0·5 µg OCP/kg/day plus 0·5 mg progesterone/kg/day in 1·0 ml CSO for the next 5 days, 1·0 mg progesterone /kg/day in 1·0 ml CSO for the next 8 days and 1·0 ml CSO alone