This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by QUINN, P. J. Articles by WHITE, I. G. Search for Related Content PubMed PubMed Citation Articles by QUINN, P. J. Articles by WHITE, I. G.

DISTRIBUTION OF ADENOSINETRIPHOSPHATASE ACTIVITY IN RAM AND BULL SPERMATOZOA

The active transport of sodium and potassium has been demonstrated in dog spermatozoa (Quinn & White, 1967) and the existence of a cation pump, involving a sodium—potassium activated ATPase has been postulated to account for the gradients of alkali metal ions between the cell and seminal plasma (Quinn, White & Wirrick, 1965). Recently, Uesugi & Yamazoe (1966) have reported the occurrence of a sodium—potassium activated ATPase in boar epididymal spermatozoa, and the present experiments were undertaken to determine the activity and distribution of ATPases in ram and bull spermatozoa.

Semen was diluted with 2·5 vol of 250 mM-sucrose, centrifuged, and the spermatozoa washed twice with 5-ml aliquots of sucrose. Washed spermatozoa, resuspended in sucrose buffered to pH 7·4 (37° C) with 50 mM-tris (tris [hydroxylmethyl] amino methane) and 40 mM