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FIXATION OF CARBON DIOXIDE BY RAM SPERMATOZOA

As the effect of carbon dioxide on mammalian spermatozoa is of current interest (Lodge & Salisbury, 1963; Hammer & Williams, 1964; VanDemark, Koyama & Lodge, 1965; Wales & O'Shea, 1966; Wales & Restall, 1966), the possibility of its fixation by ram spermatozoa has been investigated.

Aliquots (0·4 ml) of ram semen or twice-washed spermatozoa (5·4 x 10⁸ cells/ flask) were incubated at 37° C with [¹⁴C]sodium bicarbonate (1 µmole and 25 µC) in phosphate-buffered saline containing 1 mM-potassium chloride, 2mM-magnesium chloride, 10 mM-sodium Pyruvate, 30 mg/100 ml penicillin and 50 mg/100 ml streptomycin. After 15 or 60 min, 0·5 ml of the incubated semen was removed and stored at -15° C for subsequent chromatography. The remaining 0·5 ml was acidified (0·1 ml of 6 N-H₂