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Journal of Reproduction and Fertility (1967) 14 155-157
DOI: 10.1530/jrf.0.0140155
Copyright © 1967 Society for Reproduction and Fertility
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AUTOMATIC COUNTING OF SPERMATOZOA IN RABBIT SEMEN

J. E. KILHSTRÖM and D. FJELLSTRÖM

Electronic methods have been used for automatic counting of spermatozoa in semen from bulls (Glover & Phipps, 1962; Iversen, 1964), guinea-pigs (Laurence & Carpuk, 1963) and man (Segal & Laurence, 1962). The main obstacle to automatic counting of rabbit spermatozoa is the high degree of contamination of rabbit semen with granular particles, many of them being of the same size as the spermatozoa. In addition, some semen specimens contain a gelatinous substance which is sometimes difficult to remove completely by mechanical treatment. In spite of these difficulties Fowler & Hellman (1965) have obtained fairly good results in counting rabbit spermatozoa automatically. However, their method does not permit the discrimination between spermatozoa and other particles of about the same volume. The present communication gives the results obtained using an electronic cell counter for counting rabbit spermatozoa after the irrelevant particles







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Copyright © 1967 by the Society for Reproduction and Fertility.