Reproduction  
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS  
Reproduction (2008) 135 771-784
DOI: 10.1530/REP-07-0479
Copyright © 2008 Society for Reproduction and Fertility
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Data
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (4)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Izadyar, F.
Right arrow Articles by Silva, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Izadyar, F.
Right arrow Articles by Silva, F.

RESEARCH

Generation of multipotent cell lines from a distinct population of male germ line stem cells

Fariborz Izadyar1, Francis Pau1, Joel Marh1, Natalia Slepko2, Tracy Wang1, Rafael Gonzalez2, Thomas Ramos1, Kyle Howerton1, Chauncey Sayre1 and Francisco Silva2

1 PrimeGen Biotech LLC and 2 New Stem Biosciences, 213 Technology Drive, Irvine, California 92618, USA

Correspondence should be addressed to F Izadyar; Email: fizadyar{at}primegenbiotech.com) www.primegenbiotech.com

Spermatogonial stem cells (SSCs) maintain spermatogenesis by self-renewal and generation of spermatogonia committed to differentiation. Under certain in vitro conditions, SSCs from both neonatal and adult mouse testis can reportedly generate multipotent germ cell (mGC) lines that have characteristics and differentiation potential similar to embryonic stem (ES) cells. However, mGCs generated in different laboratories showed different germ cell characteristics, i.e., some retain their SSC properties and some have lost them completely. This raises an important question: whether mGC lines have been generated from different subpopulations in the mouse testes. To unambiguously identify and track germ line stem cells, we utilized a transgenic mouse model expressing green fluorescence protein under the control of a germ cell-specific Pou5f1 (Oct4) promoter. We found two distinct populations among the germ line stem cells with regard to their expression of transcription factor Pou5f1 and c-Kit receptor. Only the POU5F1+/c-Kit+ subset of mouse germ line stem cells, when isolated from either neonatal or adult testes and cultured in a complex mixture of growth factors, generates cell lines that express pluripotent ES markers, i.e., Pou5f1, Nanog, Sox2, Rex1, Dppa5, SSEA-1, and alkaline phosphatase, exhibit high telomerase activity, and differentiate into multiple lineages, including beating cardiomyocytes, neural cells, and chondrocytes. These data clearly show the existence of two distinct populations within germ line stem cells: one destined to become SSC and the other with the ability to generate multipotent cell lines with some pluripotent characteristics. These findings raise interesting questions about the relativity of pluripotency and the plasticity of germ line stem cells.




This article has been cited by other articles:


Home page
 Biol. Reprod.Home page
S. Takashima, M. Takehashi, J. Lee, S. Chuma, M. Okano, K. Hata, I. Suetake, N. Nakatsuji, H. Miyoshi, S. Tajima, et al.
Abnormal DNA Methyltransferase Expression in Mouse Germline Stem Cells Results in Spermatogenic Defects
Biol Reprod, July 1, 2009; 81(1): 155 - 164.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
U. Zechner, J. Nolte, M. Wolf, K. Shirneshan, N. E. Hajj, D. Weise, B. Kaltwasser, A. Zovoilis, T. Haaf, and W. Engel
Comparative methylation profiles and telomerase biology of mouse multipotent adult germline stem cells and embryonic stem cells
Mol. Hum. Reprod., June 1, 2009; 15(6): 345 - 353.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
C. B. Maki, J. Pacchiarotti, T. Ramos, M. Pascual, J. Pham, J. Kinjo, S. Anorve, and F. Izadyar
Phenotypic and molecular characterization of spermatogonial stem cells in adult primate testes
Hum. Reprod., June 1, 2009; 24(6): 1480 - 1491.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
J. Lee, M. Kanatsu-Shinohara, N. Ogonuki, H. Miki, K. Inoue, T. Morimoto, H. Morimoto, A. Ogura, and T. Shinohara
Heritable Imprinting Defect Caused by Epigenetic Abnormalities in Mouse Spermatogonial Stem Cells
Biol Reprod, March 1, 2009; 80(3): 518 - 527.
[Abstract] [Full Text] [PDF]

Home page
 Cold Spring Harb Symp Quant BiolHome page
M. Kanatsu-Shinohara, M. Takehashi, and T. Shinohara
Brief History, Pitfalls, and Prospects of Mammalian Spermatogonial Stem Cell Research
Cold Spring Harb Symp Quant Biol, November 6, 2008; (2008) sqb.2008.73.033v1.
[Abstract] [PDF]

Home page
 Mol Hum ReprodHome page
A. Zovoilis, J. Nolte, N. Drusenheimer, U. Zechner, H. Hada, K. Guan, G. Hasenfuss, K. Nayernia, and W. Engel
Multipotent adult germline stem cells and embryonic stem cells have similar microRNA profiles
Mol. Hum. Reprod., September 1, 2008; 14(9): 521 - 529.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
J. Yu and J. A. Thomson
Pluripotent stem cell lines
Genes & Dev., August 1, 2008; 22(15): 1987 - 1997.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS  
Copyright © 2008 by the Society for Reproduction and Fertility.