This Article Full Text Full Text (PDF) Supplementary Data Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (1) Citing Articles via Google Scholar Google Scholar Articles by Mitko, K. Articles by Bauersachs, S. Search for Related Content PubMed PubMed Citation Articles by Mitko, K. Articles by Bauersachs, S.

Dynamic changes in messenger RNA profiles of bovine endometrium during the oestrous cycle

¹ Institute of Molecular Animal Breeding and Biotechnology and ² Laboratory for Functional Genome Analysis (LAFUGA), Gene Center, LMU Munich, Feodor-Lynen-Strasse 25, 81377 Munich, Germany³ Physiology-Weihenstephan, Technical University of Munich, Weihenstephaner Berg 3, 85354 Freising, Germany⁴ Bavarian Research Centre for Biology of Reproduction, Hackerstr. 27, 85764 Oberschleissheim, Germany and⁵ Institute of Veterinary Anatomy, Histology and Embryology, LMU Munich, Veterinärstr. 13, 80539 Munich, Germany

Correspondence should be addressed to S Bauersachs; Email: bsachs{at}lmb.uni-muenchen.de

During the oestrous cycle, the bovine endometrium exhibits characteristic morphological and functional changes, which are mainly induced by progesterone (P₄), oestrogens and oxytocin. We studied the response of the endometrium to this changing hormonal environment at the transcriptome level using a custom-made cDNA microarray. Endometrium samples were recovered from Simmental heifers on days 0 (oestrus), 3.5 (metoestrus), 12 (dioestrus) and 18. The latter group was divided into animals with high (late dioestrus) and low P₄ levels (preoestrus). Significance analysis of microarrays revealed 269 genes exhibiting significant changes in their transcript levels during the oestrous cycle in distinct temporal patterns. Two major types of expression profiles were observed, which showed the highest mRNA levels during the oestrus phase or the highest levels during the luteal phase respectively. A minor group of genes exhibited the highest mRNA levels on day 3.5. Gene ontology (GO) analyses revealed GO categories related to extracellular matrix remodelling, transport, and cell growth and morphogenesis enriched at oestrus, whereas immune response and particular metabolic pathways were overrepresented at dioestrus. Generation of gene interaction networks uncovered the genes possibly involved in endometrial remodelling (e.g. collagen genes, TNC, SPARC, MMP2, MEP1B, TIMP1, TIMP2, HTRA1), regulation of angiogenesis (e.g. ANGPTL2, TEK, NPY, AGT, EPAS1, KLF5 ), regulation of invasive growth (e.g. PCSK5, tight junction proteins, GRP, LGALS1, ANXA2, NOV, PLAT, MET, TDGF1, CST6, ITGB4), cell adhesion (e.g. MUC16, LGALS3BP) and embryo feeding (e.g. SLC1A1, SLC11A2, SLC16A1, SEPP1, ENPP1). Localisation of mRNA expression in the endometrium was analysed for CLDN4, CLDN10, TJP1, PCSK5, MAGED1, and LGALS1.

This article has been cited by other articles:

				S. Bauersachs, S. E. Ulbrich, V. Zakhartchenko, M. Minten, M. Reichenbach, H.-D. Reichenbach, H. Blum, T. E. Spencer, and E. Wolf The endometrium responds differently to cloned versus fertilized embryos PNAS, April 7, 2009; 106(14): 5681 - 5686. [Abstract] [Full Text] [PDF]

				M. C. Satterfield, K. Hayashi, G. Song, S. G. Black, F. W. Bazer, and T. E. Spencer Progesterone Regulates FGF10, MET, IGFBP1, and IGFBP3 in the Endometrium of the Ovine Uterus Biol Reprod, December 1, 2008; 79(6): 1226 - 1236. [Abstract] [Full Text] [PDF]

				I. Hue and J.-P. Renard Focus on mammalian embryogenomics Reproduction, February 1, 2008; 135(2): 117 - 118. [Full Text] [PDF]