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A microarray for microRNA profiling in mouse testis tissues

¹ Division of Human Morbid Genomics, State Key Laboratory of Biotherapy, Department of Medical Genetics, West China Hospital, Sichuan University, Renmin Nanlu, Section 3, No. 17, Chengdu 610041, People’s Republic of China and ² Laboratory of Ophthalmology, West China Hospital, Sichuan University, Chengdu 610041, People’s Republic of China

Correspondence should be addressed to Y Ma; Email: mayongxing{at}263.net

MicroRNAs (miRNAs) are short non-coding RNA molecules playing regulatory roles by repressing translation or cleaving RNA transcripts. Recent studies indicate that miRNAs are mechanistically involved in the development of mammalian spermatogenesis. However, little work has been done to compare the miRNA expression patterns between immature and mature mouse testes. Here, we employed a miRNA microarray to detect 892 miRNAs in order to evaluate the expression patterns of miRNA. The expression of 19 miRNAs was significantly different between immature and mature individuals. Fourteen miRNAs were significantly upregulated and five miRNAs were downregulated in immature mice and this result was further confirmed by a quantitative real-time RT-PCR assay. Many target genes involved in spermatogenesis are predicted by MiRscan performing miRNA target scanning. Our data indicated specific miRNAs expression in immature mouse testis and suggested that miRNAs have a role in regulating spermatogenesis.

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