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Bovine germinal vesicle oocyte and cumulus cell proteomics

¹ Department of Animal and Dairy Sciences, Mississippi State University, Starkville, Mississippi 39762-6100, USA, ² Mississippi Agricultural and Forestry Experiment Station, Starkville, Mississippi 39762, USA, ³ College of Veterinary Medicine and ⁴ Institute for Digital Biology, Mississippi State University, Starkville, Mississippi 39762, USA and ⁵ Department of Reproduction and Artificial Insemination, Uludag University Veterinary Faculty, Gorukle-Bursa 16059, Turkey

Correspondence should be addressed to E Memili; Email: em149{at}ads.msstate.edu

Germinal vesicle (GV) breakdown is fundamental for maturation of fully grown, developmentally competent, mammalian oocytes. Bidirectional communication between oocytes and surrounding cumulus cells (CC) is essential for maturation of a competent oocyte. However, neither the factors involved in this communication nor the mechanisms of their actions are well defined. Here, we define the proteomes of GV oocytes and their surrounding CC, including membrane proteins, using proteomics in a bovine model. We found that 4395 proteins were expressed in the CC and 1092 proteins were expressed in oocytes. Further, 858 proteins were common to both the CC and the oocytes. This first comprehensive proteome analysis of bovine oocytes and CC not only provides a foundation for signaling and cell physiology at the GV stage of oocyte development, but are also valuable for comparative studies of other stages of oocyte development at the molecular level. Furthermore, some of these proteins may represent molecular biomarkers for developmental potential of oocytes.